Advanced HX-MS² Epitope Mapping Service
Antibody-antigen epitope mapping, protein-ligand interaction analysis
Fast
Using novel DIA-based HX-MS² methodology, sample handling automation and proprietary data processing software, projects are run in one week or less.
Sensitive
An epitope map requires no more than 10 of antigen and antibody.
Economical
Automation of data analysis allows us to have the lowest-cost epitope mapping service in the market.
Single antibody-antigen epitope analysis
Analysis time:
One week from sample to report.
$7500 CAD
Hydrogen-deuterium exchange mass spectrometry (HDX-MS), also called HX-MS, is a powerful method for identifying residues involved in an antigen-antibody interaction. The method is based on exchange of amide hydrogens in protein for deuterium, a process dependent on solvent accessibility and local structure. A differential analysis of bound and unbound states of an antibody-antigen pair can localize protein binding events and their allosteric effects. The method can identify both linear and conformation epitopes with resolution dependent on protease digestion with, 3-6 amino acid resolution routinely achieved.
Our recent advancements in HX-MS² enable unparalleled speed and accuracy of analysis. The results are interpreted in a professional report and mapped to structure, if one is provided. Interpretation of the data and identification of the epitope (or paratope) is also provided.
High volume projects
Upon consultation
Detailed interaction interfaces mapped to protein structure, just around the corner.
Features of HX-MS epitope mapping
Native Conditions
HX-MS² tolerates the vast majority of protein buffers, salts, small molecule ligands and other proteins as impurities.
High Resolution
Digestion of sample proteins by high-efficiency proteases offers detailed sequence maps, with high resolution achieved by peptides and their overlaps.
Dynamic States
Proteins are analyzed in their native state in solution. HX-MS² integrates all dynamic and structural states present, offering maximum result relevancy.
Speed to Reporting
24/7 automation for acquisition and data analysis offers unparalleled consistency and throughput for HX-MS². Professional report and full confidentiality are a standard.
High sensitivity
HX-MS² offers industry-leading sensitivity. Only 10 μg of antigen and antibody is needed for a full analysis, and research-grade modes only require 10 ng!
Expertise
We are pioneers in HX-MS², with years of experience in HDX-MS. We can tackle a multitude of protein systems and optimize each analysis for maximum results.