Ultra Rapid HDX-MS² Epitope Mapping Service
Custom antibody-antigen epitope and paratope mapping in 1 week !
RAPID TURNOVER
Using novel DIA-based HDX-MS² methodology, sample handling automation and proprietary data processing software, as little as 1 week project turnaround times are possible.
LOW SAMPLE CONSUMPTION
For a thorough epitope and paratope mapping, as little as 100 μg of each antigen and antibody are needed.
LOW COST
Automating both data acquisition and data analysis allows us to have the lowest HDX-MS epitope mapping analysis prices on the market.
Single Antibody-Antigen Pair Epitope and Paratope analysis
Analysis time:
One week from analysis start to result report.
$8500 CAD
Hydrogen-deuterium exchange mass spectrometry (HDX-MS), also called HX-MS is a powerful method for identification of residues involved in an antigen-antibody interaction. The method is based on exchange of amide hydrogens in protein for deuterium, a process dependent on solvent accessibility and local structure (especially hydrogen bonding). This allows a differential analysis of bound and unbound states of antibody-antigen pair to detect and precisely localize protein binding events. The method can identify both linear and conformation epitopes with resolution dependent on protease digestion with 3-6 amino acid residue resolution typically achieved.
Our recent advances is data acquisition (novel DIA-based HDX-MS acquisition) and corresponding data processing software (fully automated data validation with our proprietary AutoHX software) enable unparalleled speed and precision of our HDX-MS analysis at lowest cost on the market.
The results are visualized as a deuteration differences across the protein sequence or on a model structure if one is provided. Interpretation of the data and identification of the epitope (or paratope) is also provided.
High-Throughput Epitope and Paratope Analysis (≥5 pairs)
Analysis time:
Two weeks from analysis start for each 5 antigen-antibody pairs analyzed.
$25 000+ CAD
Detailed interaction interfaces mapped to protein structure just around the corner.
Features of HDX-MS based epitope mapping
Native Conditions
HDX-MS tolerates vast majority of typical protein buffers, presence of salts, small molecule ligands and other proteins as impurities.
High Resolution
Digestion of sample proteins by high-efficiency unspecific proteases offers detailed sequence maps with high resolution given by peptides and their overlaps.
Dynamic States
Proteins are analyzed in their native state in solution and the method captures a sum of all dynamic and structural states present offering maximum result relevancy.
Rapid Turnaround Times
Sampling automation for 24/7 data acquisition and novel DIA-based methodology allowing automated data analysis offers unparalleled consistency and throughput in HDX-MS.
High sensitivity
Mass spectrometry is inherently a sensitive method making 100 μg of antigen and antibody enough for acquisition of a high-quality epitope and paratope maps.
Expertise
With years of experience in HDX-MS, we are able to tackle multitude of different protein systems and optimize each analysis for maximum results. Professional report and full confidentiality where needed are a standard.